in what basis wavelength will select for a compound at initial method developm

in what basis wavelength will select for a compound at
initial method development trials

Question Posted / kishan05

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in what basis wavelength will select for a compound at initial method development trials..

Answer / ramkrishna thakare

1 ) for any unknown compound firstly solubility check.
2 ) then on the basis of solubility UV - scanning should
be done

3 ) on the basis of UV - scanning Maxima must be
checked.& then on the basis MAXIMA wavelength should be
selected.it may multiwavelength or single-wavelength maxima .

RAMKRISHNA THAKARE -------SANDOZ

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in what basis wavelength will select for a compound at initial method development trials..

Answer / u.venkatramireddy

Based on Chromophore in the compound

posted by uvreddy
DrReddy's

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in what basis wavelength will select for a compound at initial method development trials..

Answer / vijay

Based on wavelength of the compound. Not only lambda max we have to consider co-elute peak, cut off value of solvent.

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in what basis wavelength will select for a compound at initial method development trials..

Answer / shami thakur

Not mentioned type of method development UV or HPLC
During hplc method development mobile phase are selected according to dissociation constant value and all study carried with the help of PDA detector wavelength range 200-400nm

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