MICROBIOLOGICAL ENGINEERING - QUESTION 28.1 : In the calculation of the growth of bacteria, colony forming unit (CFU) in serial dilution is used. In a laboratory, viable count assay is used to estimate CFU. Formula applied is CFU / mL = (number of colonies x dilution) / (amount plated, in unit mL). Acceptable plate count is either between 20 and 200 or between 30 and 300 according to 2 different references. A wastewater sample of 200 ml is added to and mixed with 1.8 L of sterile water. Another 200 ml of the mixture is added to and mixed with 1.8 L of sterile water. (a) Calculate the dilution of first mixture and the dilution of the second mixture. (b) 100 microlitres of wastewater samples from the first mixture and the second mixture are placed separately on 2 different alga plates. The first plate has 250 colonies and the second plate has 23 colonies. Calculate the average CFU / mL.

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Any idea on recombinant protein expression?

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Tell us about a time when you failed to meet a deadline. What were the repercussions?

4 Answers   Bhavani Engineering Works, Caltex, Emirates, Holiday Inn, Regent, United States Seafoods,

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Calculate the line size for a cooling water line with a volumetric flowrate of 1200m3/h of water. Density of water = 1000kg/m3

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Dear guys we adding hypo at climax plant and that water is inlet to DM plant so with hypo water that can affect my resins of cation and anion. if it is affect life of my anion and cation so what kind of steps should be take.

0 Answers   Hindustan Unilever Limited,

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What's the difference between superphosphate and triple super phosphate fertilizer?

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QUANTUM BIOLOGY - EXAMPLE 33.10 : The wavefunction starts out in a superposition of possible states in a closed black box like this : Ψ (kitty) = 0.7071 Ψ (alive) + 0.7071 Ψ (dead) where Ψ (kitty) = wavefunction of a kitten, Ψ (alive) = wavefunction of a living kitten, Ψ (dead) = wavefunction of a dead kitten. By prediction and calculation, find the probability of : (a) a living kitten inside the black box; (b) a dead kitten inside the black box.

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wha is Adsorption column chromatography?

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How can arsenic be removed from aqueous waste streams?

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A cantilever beam of length L = 3 m carries a uniformly distributed load (UDL) of W = 20 N / m throughout the length. Calculate the bending moment, BM of the beam near the fixed end. What is the shear force, SF at this point? Let SF = -WL, BM = -LWL/2.

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What are the main terms in unit operations? What are its characteristics?

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if the length of a pipe doubled what will be the effect on Pressure in line?

7 Answers   ISRO,

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