Generally for Normal Phase HPLC,stationary phase is Polar.In
place of polar if we take non-polar means what happened & why?
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Answer / santosh
In normal phase , stationary phase is polar while mobile
phase is non polar & separation is achieve by the polarity
of the compound. More polar compound eluted out last while
non polar compound eluted fast
| Is This Answer Correct ? | 20 Yes | 4 No |
Answer / sri
Supose if we take non polar stationary phase and non polar
mobile phase bonded phase will lose.becase non polar
stationary phse dissolved in non polar mobile phase.
| Is This Answer Correct ? | 11 Yes | 0 No |
Answer / umesh patel
in case of normal phase chromatograpy, as we are using
highly non polar sovent as mobile phase ,it will dissolve
the column bed of non polar SP
like dissolves like
| Is This Answer Correct ? | 7 Yes | 3 No |
Answer / nagaraj
in normal phase chromatography is used for seperation of
compounds which are having non polar segments .if we take
non polar asorption can acheived against separtion.because
it is seperation chromatogrphy.
| Is This Answer Correct ? | 1 Yes | 1 No |
Answer / ch s raju
in normal phase we are using non polar solvent as a mobile
phase and if we are using non-polar stationary phase it
dissolves in non-polar solvent column collapes
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / amol
In normal phase , stationary phase is polar while mobile
phase is non polar & separation is achieve by the polarity
of the compound. More polar compound eluted out first while
non polar compound eluted last . if we take non polar
stationary phase it will happen reverse..
| Is This Answer Correct ? | 5 Yes | 20 No |
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