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I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
Hi, I am Gurunathan. I am in need of ONGC, IOCL, DRDO technical test ( chemical engineering)and general aptitude questions. If anyone has kindly post it to me soon.
i qualified karnataka bank clerical exam, next month 15-4-2017 may be interview there..... suggest me some interview tips andtell me the expected question to be asked in the Karnataka bankclerical exam plz reply to my mail.... meghas.madhu25@gmail.com
Sir,In our company there are 3 caterpillar make(capacity:(1) 3.3KV,2.5MW(2)3.3KV,2.5MW & (3)3.3KV,1.9MW) & 1 deutz make (capacity:11KV,3.8MW)gas engines utilised,all are running in paralell. There are three transformer utilised,(1) 6.25MVA,3.3KV/22KV,Impedence voltage:6.7% used for two generator,both are 2.5MW(2)5.5MVA,3.3KV/22KV,Impedence voltage:7.2% used for one generator of 2.5MW & (3) 5.5MVA,11KV/22KV,Impedence voltage:7.8% used for one generator of 2.5MW.all generator breakers capacity of 1250Amps. Sir,I want the details of fault level calculation of generator,transformer individually & for whole system. If you require any information from me.feel free for contact me sir.pl.send the answer of my question as the earliest as possible.
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it is better to invest in which securities short term/long term or both? please reason4 the same
